Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISYEKTDMNHGTFPVFDGHND--VLTRLWLSDHNNPAQAFIQEGLAGHLDLKRCQQAGFVGGMF-AIFLPPFHYVKQHHPNKLFDQDASDFTQQQIEHICLAQLDLAKQLAE-YSNDIQICTTVQDIQHCLTQQKLAIILHMEGAEALQHNPDLLDVFYDAGLRSIGPLWNRPSRFGHGLNAKFPHSPDTGAGLTNEGKAFIKRCADKKMVIDVSHMNERAFWDTANILQQPIVATHSNVHALCPQARNLTDNQLKAIKDSKGIVGLNFDVAFLRKDGQRDAN-TSIEVLLEHLEYLIDQMGENHVGFGSDFDGALISHE-IEDVRGLHLLIEAMQKRHYSNEIIEKICFSNWLTVLNRILDK 5LX1 Chain:A ((30-356)) -------------VPLIDGHNDFPYMIRGWFRNDINGQDAHLYDMPIGQTDLQRLQK-GLLGGQFWSAFVPC-----PKNPDK-------EVGSLEALRQTLQQLDVIHRLIERHPTILQFADSAASIWSSFRAGRVASLIGIEGLHQIADSVSALRMLHRLGVRYVTLTHNCHNAFADAATV----SPELHGGLSRKGERLIRELNRMGMMIDLSHTSHEAQTQALRLSRAPVIYSHSSIYSLRAHARNVTDENLHLLHRNRGVVM----ICFLRELLASEADQATLAHVIDHIIYAGTRIGYEHVGIGSAFDGMLRGPDGLHDVSCYPALVAGLLERGVSEEDVKRVMGLNVIRVLEEV---

General information: TITO was launched using: RESULT: Template: 5LX1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1988 -9877 -4.97 -30.77 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -4.97 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_5LX1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LX1-query.scw PDB file : Tito_Scwrl_5LX1.pdb: