Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MR---KILGIASLIMSTGVVHAEQLNEQEQISPYSVNVTFASQYISRGFQQTWGKPALQIGLDYANPNGLFVGTWASNVSSNYLRDASVEWDFYAGYLKTIDKFSIGMSVYYYYYPGAKSTPETGSTNYNYGEIVPQIGYGPLSLKYFITYTPDYAGYNSNTMGGPEGKRSRGTTYLDLNFTQPINESWTFGAHYGYERIKNFSEANFQDMKVELIKDLGDGWTTGLAYTKAWDKDGYYRNYSNGESDAPISNPIDSTFTVSVKKVF 2KIX Chain:A ((1-33)) MLEPFQILSISSFILSALHFIAWTIGHLNQIKR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2KIX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 8 -470 -58.69 -15.65 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : -58.69 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_2KIX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KIX-query.scw PDB file : Tito_Scwrl_2KIX.pdb: