Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------MMTEQKSALSLRYFLN-----------LEESQDGFGLVTFGKKQFTRFLTPVISIAIIIWG--------------LYLGF-SGVGRYYVALGVFFLVM---QGLMRYWLLPMMFK----RQFVRYQFGKSEQGIDLYQDYFE----FYAAGRK---QAVQYSEVQTFAKGKLTYMLELKNK----TVIIVPKRAFAQASDQTIFENTFKK 1E42 Chain:A ((26-258)) GGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN

General information: TITO was launched using: RESULT: Template: 1E42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 639 -28171 -44.09 -169.70 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -44.09 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_1E42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E42-query.scw PDB file : Tito_Scwrl_1E42.pdb: