Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYRHHFHAGNFADVMKHVLLLQLLNRLNAKDKPYRYIDTHGGAGKYDLSQAPAQKSGEFLTGIHRLVQLSGMEKRQAPEAIQQYLKLVEELRAQEGKGSYPGSPWFALQGMREIDKATIFEMQRDVFQQLRHNIHD---KRAGLHERDAYEGLLAVIPPKEKRGLVMIDPPYELERKD---------FPQLVELLQSAYKKWPTGVFAVWYPIKDRAMIERFEKKMFKTGIRRQLICEICVWPDDTPVGLNGCGLLVINPPWQFSEQADQALQWLFPHLRMQETGGHAAVRWLVGE 1WXX Chain:C ((211-329)) ----------------------------------------------------------------------------------------------ERALDVFSYAGGFALHLALGFREVVAVDSSAEALRRAEENARLNGLGNVRVLEANAFDLLRRLEKEGERFDLVVLDPPAFAK-GKKDVERAYRAYKEVNLRAIKLLK--EGGILATASCSHH---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1WXX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 443 -10575 -23.87 -98.83 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.59 3D Compatibility (PKB) : -23.87 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_1WXX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WXX-query.scw PDB file : Tito_Scwrl_1WXX.pdb: