Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTFTLFFGVPSGCSFGSIVALEWSGLPYQLCRIEMPAQCNTEEYRQINPVGETPALLYDNGKVLTESMAILNHIMAKSLNAAFHYEQGTEQFNLFNQKLAFLNTTLFNAFSPHWYSLEHSSSEGNKQALREYGAVQVKKAFTALENSLTNQTWLMGDQLSFVDGYYAGIARWLKYHNVIDIDQFPNCKRLYEQLQNEAAVKFAHAIEQE 3MAK Chain:A ((43-185)) -------------------------------------------EFLKINPQHTIPTLV-DNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFK-----KIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAK-FEISKYANVNRWYE-----------------

General information: TITO was launched using: RESULT: Template: 3MAK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 -78303 -150.87 -547.57 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -150.87 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_3MAK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MAK-query.scw PDB file : Tito_Scwrl_3MAK.pdb: