Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAANKILLQKGFCAEMEKYTRYKELYMSLPLINKQFMKQGLAITIATMKMNVTHATTEPQEIQQLGTDLNALFFNEKEKGTDSRVMTMACYSL 1FP4 Chain:B ((128-173)) ---------------------------------QQNMKDGLQNCKATYKPDMIAVSTTCMA-EVIGDDLNAFINNSKKEG-------------

General information: TITO was launched using: RESULT: Template: 1FP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 84 179 2.13 3.88 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 2.13 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_1FP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FP4-query.scw PDB file : Tito_Scwrl_1FP4.pdb: