Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWLSNEGGFYMKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAAGNLNQNVGRAQWEPQSIEATK----NTFIDGKIASAGVSGFEPLD---EQTSNRWHKNMVNPG-TLNITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELIT--KIDDKATVPPKTVNQTITLPQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ 5FTZ Chain:A ((1-172)) ------------------------------------HGYTDLPVSRQKV---CQNGTVGGCGAIQWEPQSVEGPKGFPASGPADGTICSAGHGSFAALDSPKQPNGQAWPTTRVNGGQSYTFRWQFTARHATTDFKYYVTKPGWNQNHNLARSDLNLTPFFTVPYGGKQPPATLSHSGTLPSGLSGHHVILAVWTVHDTGNAFYACSDVTF-

General information: TITO was launched using: RESULT: Template: 5FTZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 796 29615 37.20 182.81 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 37.20 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_5FTZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FTZ-query.scw PDB file : Tito_Scwrl_5FTZ.pdb: