Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITSDIKNADEYVRKVDKSQIPTCNIMGVNIAAINMEWLLEYLEKNIDLLHGDYICVSNVHTTVTSFEEPDYCAVQNGGIMAIPDGGPLSTVGRKRGYKNMERTTGPSLMGKIFKVTNEKGYRHFFYGSKQET--------------------LDLLEEKLKVYYP--GIQIAGM-YSPPFRPLTEEEDKAVIKMINAVKPDFVWIGLGAPKQEKWMAEHQGKIDGLMIGVGAGFDYYAGNIQRAPQWMQKWNLEWMYRLIQDPKRLFYRYWHTNTKFILNAVIRRK 5JD3 Chain:F ((24-113)) ---------------------------------------------------------------------------------------------------------------------FAAGSKLVIIGDSITDAGRDKGIGGEGLFNAHGSGYVALLNAHLFARFPERRLRLVNQGNSGNTV---RDLAARWQNDVFGLKPDYVAMMIGI-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JD3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 246 736 2.99 10.99 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain F : 0.54 3D Compatibility (PKB) : 2.99 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_5JD3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JD3-query.scw PDB file : Tito_Scwrl_5JD3.pdb: