Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAE---DKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPL--T---V-NLDFQRNNKTVFKASSF----AGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKT----KILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPF-----------------------------F-------------LDD---RRTPAKMCLNRT----------------SQENISFETMYDVLSTK----P-----------VL------NKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 5IMG Chain:D ((7-329)) --------------------------------------------------------------------------------------------------------------------------------------------GSATSILVGKKASIDGSTLISRNDDGHEAL-D--PQRFVVVNPEDQPRDYTSVISKVNVKLPDDPQRYTSIPNSILTNGIWPA-AGINSSNVAMSATETITTNSRVQ--GLD-PFVENGLGEEDLVTVVLPYVKSAREGVKRLGSLLEEYGTYEPNGISFA-DN-EEVWWLETIGGHHWAAVRIP---DDAYVVAPNR--MNIDQFDFDSDDTLCSSDLKDLIDNNNLNPDFENYNLRHIFGSASIKDTVYNNPRTWYGQKFFSPDDTADDPMEQDLPFICHANRKISVEDVKFVLSSHFENTKYDVYGSGSQSDKTLFRPIGINRNHNVHILQIRN-----------------

General information: TITO was launched using: RESULT: Template: 5IMG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1255 43225 34.44 192.97 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.56 3D Compatibility (PKB) : 34.44 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_5IMG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IMG-query.scw PDB file : Tito_Scwrl_5IMG.pdb: