Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG 3WTP Chain:B ((25-102)) -------------------------NIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

General information: TITO was launched using: RESULT: Template: 3WTP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 174 -24757 -142.28 -317.40 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain B : 0.91 3D Compatibility (PKB) : -142.28 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_3WTP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WTP-query.scw PDB file : Tito_Scwrl_3WTP.pdb: