Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWN-TLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTL--DGGDIINALCFSPN-RYWLCAATGPSIKIWDL-EGKIIVDELKQEVISTSSKAEPPQCTSLAWSADG-QTLFAGYTDNLVRVWQVTIGTR 2G99 Chain:A ((4-262)) ----------------------------------------------PVKPNYAL-KFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLK--GHSNYVFCCNFNPQSN--LIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLK-------TYTGHKNEK-YCIFANFSVTGGKWIVSGSEDNLVYIWNL-----

General information: TITO was launched using: RESULT: Template: 2G99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1433 -23492 -16.39 -92.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -16.39 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2G99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G99-query.scw PDB file : Tito_Scwrl_2G99.pdb: