Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAF--QPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQL-LEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD 2AMF Chain:A ((4-259)) MKIGIIGVGKMASAIIKGLKQ----TPHELIISGSSLERSKEIA-EQLALPYAMSHQDLIDQVDLVILGIKPQLFETVLKPL----HFKQPIISMAAGISL----QRLATFVGQDLP-LLRIMPNMNAQILQSSTAL-TGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTALAGSSPAYIYLFIEALAKAGVKNGIPKAKALEIVTQTVLASASNLKTSSQSPHDFIDAICSPGGTTIAGLMELERLGLTATVSSAIDKTIDKAKSL----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2AMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1047 -116803 -111.56 -461.67 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -111.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_2AMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AMF-query.scw PDB file : Tito_Scwrl_2AMF.pdb: