Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------MTYSHIYQVLFLPKLSIKRWHFLGLVLVNFALSYLSHFELFM----VQWKHVIQII---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5LTE Chain:A ((5-337)) FKPLESYREDFSFRNSPAAIARFPFPFPEDQYMYSVNLEPAVSRDPGSVFEHQFDVDEHYVSEMAERARVLELDPGRCLVMPHMAQAAWDTLAMLMEHLARDYPQHFRLTRQGDAWHWQNLALGIDQRFTFGDPASLPCEPLEYITRQMQGDFAVLDQRDGDLFMDAGMVTCPADWSLRFDAGMSFKQWHSPVPMAHQMGVFDRALKYLLNIQVGAPVRRLNWTLTINPRLDTSPETYHEWGNDRGKVTPDNVGRLVHLRVELQLMARLPRSNALLFGIRTYLISLDELVSNPAWAQRLHRVMRDLPDPIADYKGITRYRQTLVDWLRRFDPE

General information: TITO was launched using: RESULT: Template: 5LTE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 62 -6475 -104.44 -124.52 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -104.44 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.017

(partial model without unconserved sides chains): PDB file : Tito_5LTE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LTE-query.scw PDB file : Tito_Scwrl_5LTE.pdb: