Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTVLEAYQIVSSIHR--RNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQALRNR-TPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLAILCNYANNLANTPINPELQPYA 2PFX Chain:B ((8-188)) PTALDLPMADPLPDETQKYFEICQEKLG-MVPNVLKAYAFNVEKLNAFTAMYNDLMLGESQLSKLEREMIAVVVSSINKCFYCLVAHGAAVR-QLSGDPQLGEMLVMNYRVAPLDARQRVMLDFAAKMTRASAEIEEADREVLRSHGFNDRDIWDIANVTGFFNMTNRVASATAMMPNAEYHG--

General information: TITO was launched using: RESULT: Template: 2PFX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 750 -49021 -65.36 -275.40 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -65.36 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_2PFX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PFX-query.scw PDB file : Tito_Scwrl_2PFX.pdb: