Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVAAQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENF--LTHAKEGY-YNGITFHRVIDGFMVQTGD-PKGDGTGGQSIWHDKDKTKDKGTGFKNEITPYLYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLDGKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS 3EOV Chain:A ((26-166)) ----------------------------------------------------------------------------------GRITIGLFGKDAPLTTENFRQLCTGEHGFGYKDSIFHRVIQNFMIQGGDFTNFDGTGGKSIY--GEKFADENLNVKHFV--------GALSMANAG-PNTNGSQFFITTAPT------------------------PWLDGRHVVFGKVLDGMDVVLRIEKTKTNSHDRPVKPVKI-------------

General information: TITO was launched using: RESULT: Template: 3EOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 641 22566 35.20 164.71 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 35.20 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_3EOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EOV-query.scw PDB file : Tito_Scwrl_3EOV.pdb: