Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTGRVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRIIQERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYKYQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDTDEEYR 5F2V Chain:Y ((3-195)) ---QWERFLVPYRQAVEELKVKLKGIRTLYEYEDDHSPIEFVTGRVKPVASILEKARRKSIPLHEIE-TMQDIAGLRIMCQFVDDIQIVKEMLFARKDFTVVDQ-------------SYHLVVLYPLQTVSGEKHVLVEIQIRTLAMNFWATIEHSLNYKYSGNIPEKVKLRLQRASEAASRLDEEMSEIRGEVQEA--------------------------

General information: TITO was launched using: RESULT: Template: 5F2V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 658 -97859 -148.72 -543.66 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain Y : 0.83 3D Compatibility (PKB) : -148.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_5F2V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F2V-query.scw PDB file : Tito_Scwrl_5F2V.pdb: