Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL-AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQY--IELKNKVLRLSIGREVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQRLLDGEIKSYLF---FTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSALPYYMVPMTSLMQASYGDVSL---MKLFTPYILYIVPYMVLEKY-EDNV- 5T1O Chain:B ((2-256)) -RIRALPAAPG---------VAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSD-------NYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRD--GAANSQAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAEDDVN

General information: TITO was launched using: RESULT: Template: 5T1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1093 -70451 -64.46 -288.73 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -64.46 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.127

(partial model without unconserved sides chains): PDB file : Tito_5T1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T1O-query.scw PDB file : Tito_Scwrl_5T1O.pdb: