Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVSDVLACLDRD--V---TY-GDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDWLAKGKTSQQVLEKYEKHK 5X49 Chain:B ((67-120)) -------------------------------DNNFLYLCGFQEPDSILVLQSLPGKQLPSHKAILFV--PRRDPSRELWDGPRSGTD---------------------------------------

General information: TITO was launched using: RESULT: Template: 5X49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 87 -5602 -64.39 -116.71 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : -64.39 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_5X49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X49-query.scw PDB file : Tito_Scwrl_5X49.pdb: