Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVDRVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLLDMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGDKVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS 4U5T Chain:B ((11-32)) -----------------------------------------------------------------FLEKENTALRTEVAELRKEVGR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4U5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2 -174 -87.00 -7.91 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.50 3D Compatibility (PKB) : -87.00 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.705

(partial model without unconserved sides chains): PDB file : Tito_4U5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U5T-query.scw PDB file : Tito_Scwrl_4U5T.pdb: