Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPE--GEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDE--GHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVG-L-----------------R-HY----------------K--TP--EIQGDILLIHGEQDDITLLSDAIEWAKP-QKHPITILSGANHFFTGYLKQLRQIITRFIIMK 2WTM Chain:B ((8-247)) ---------------CDGIKLNAYLDMPKNNPEKCPLCIIIHGFTGHSE-ER--HIVAVQETLNEIGVATLRADMYG---------DHTLFKWLTNILAVVDYAKKLDFVTDIYMAGHSQGGLSVMLAAAMERD--IIKALIPLSPAAMIPEIARTGELLGLKFDPENIPDELDAWDGRKLKGNYVRVAQTIRVEDFVDKYTKPVLIVHGDQDEAVPYEASVAFSKQYKNCKLVTIPGDTHCYDHHLELVTEAVKEFMLE-

General information: TITO was launched using: RESULT: Template: 2WTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 946 -52717 -55.73 -281.91 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -55.73 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_2WTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WTM-query.scw PDB file : Tito_Scwrl_2WTM.pdb: