Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQQRNILATLGIDVWIPRTQVCQKNNAHTLWRDQVVEPHESITVPTIDVPAFEQKNTQPQVFEIPKVVEEPPIVVAEVSQPEILVEKPKVIEQETITPFELQAYCLEKCVIFVDVTALETEEKQL----WANIQKAKVGRYSELRWPFPLAAYQDQRGVGSYIQGFLDAVAAEKKILCLGKCAYIQHANIIHLASL-KEMLDKPLLKKRLWQLMQDNNE 4YH8 Chain:A ((15-194)) --KVNCSFYYKIG---------ACRHG-------ERCSRKHV--------------KPNFSQTILCPNMYKNPIH------EPNGKKFTQRELAEQFDAFYEDMFCEFSKYGEVEQLVVCDNVGDHLVGNVYVRFKYEESAQNAIDDLNSRWYSQRPVYAELSPVTDFREACCRQHET---SECQRGGLCNFMHAKKPSPQLLRDLVLAQRKYLALNAAEE

General information: TITO was launched using: RESULT: Template: 4YH8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 777 -20022 -25.77 -114.41 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -25.77 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_4YH8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YH8-query.scw PDB file : Tito_Scwrl_4YH8.pdb: