Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTANIMTTLKNQITDALKTSMRAKDMATVTVLRSL---------QAAIKQIEVDERIELDDAQVLAVIEKQIKQR------KESIKAFEGAGRDDLASKEQAEAEVLSQFLPEAMTEEELDSLIEQTIAAQEATSMKDMGKVMNSLRPIIAGRADPAQVSAKIKAKLA 3EK8 Chain:A ((337-430)) -----------------------------TVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISA---AELRHVMTNLGEKLTDEEVDEMIRE------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EK8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 126 9024 71.62 114.22 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 71.62 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3EK8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EK8-query.scw PDB file : Tito_Scwrl_3EK8.pdb: