Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLSQIDEMLIGDLDLQFVMPWEFEDLNPEAFREFNRELYA-HRFDLD-YESYSVDNDIDLHLSNY-YSSCETNKLIPSNCFFKLVEYMLVVLESR-SFTSLFSNCVEFYNTKYSNEYLTFFLNLFSTS---K-SEVA-RDNLRMYITEIFDDKRYW-ANNE-LFDDSLLFEENLQQLQIIRVM 2G3V Chain:A ((22-182)) --------KLIESLQ-------ENELLNTDEKKKIIDQIKTMHDFFKQMHTN---KGALDKVLRNYMKD---YRAVIKSIGVDKFKKVYRLLESETMELLHAIAENPNFLFSKFDRSILGIFLPFFSKPIMFKMSIREMDSQIELYGTKLPLLKLFVMTDEEMNFYANLKTIEQYNDYVRDL--

General information: TITO was launched using: RESULT: Template: 2G3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 487 4617 9.48 30.78 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 9.48 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_2G3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G3V-query.scw PDB file : Tito_Scwrl_2G3V.pdb: