Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTKQQRAELKQKFGGHCAYCGDLLGDKWHADHIEAVKRDLIHVGGGKLITGEMTRPQNDTLENMNPACVPCNKNKSSMPLEGWRKLLTHYRDVQLLRDSTHAHHLLRFGLIEIKTKPVTFFFENYKGASHE 1QHT Chain:A ((79-143)) --------------------------------------------------------------------------------PIEVWKLYFNHPQDVPAIRDRIRAH----------------------------

General information: TITO was launched using: RESULT: Template: 1QHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 17 -4126 -242.71 -165.04 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -242.71 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_1QHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QHT-query.scw PDB file : Tito_Scwrl_1QHT.pdb: