Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKNVVVLGTQWGDEGKGKIVDLLTDQAAAVVRYQGGHNAGHTLVVGGKKTVLHLIPSGILREKVLCLIGNGVVLSPAALIEEMGILEAEGVPVKERLRISPNCPLILPNHIALDQAREKKRGNAKIGTTGRGIGPAYEDKVARRAVRVADLVRGGAALEEKLTEMLELHNFQLTQFYGVEAVKFEDVLALCNQWREVLAPLVIDVTKVLHDYRKEGKAVMFEGAQGSLLDIDHGTYPYVTSSNTTAGGVSSGSGMGPLHLDYVLGITKAYTTRVGAGPFPTELVYDAASDSGDPIGKHLGTVGHEFGASTGRQRRCGWFDAEILRRSVDVNSLSGICLTKLDVLDGLEEVKICVGYENTDSGCVGSSD--AVSFECLKPIYETMPGWSESTVGLTSIDQLPANALAYVKRIEQLIECPIDIVSTGPDRAETIILRHPFSA 1GIN Chain:A ((1-431)) -GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLF-DKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGAGPFPTELF--------DETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLDVLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGLPQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA

General information: TITO was launched using: RESULT: Template: 1GIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2640 -158705 -60.12 -369.94 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -60.12 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_1GIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GIN-query.scw PDB file : Tito_Scwrl_1GIN.pdb: