Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKALPIAVAVVLGGAALVPVYYATQHPTTEVGHKADKNASPIQKISYVLGYEVAQQTPPELDTKAFVQGIHDVRNKQPSAYTQEDLKAAVAAYEKELQQKMQHQDKPEQAGTATDSADAQFLAENKTKAGVKTTASGLQYIITKEGTGKQPTAQSIVKVHYEGRLINGQVFDSSYKRGQPVEFPL--NQVIPGWTEGLQLMKEGGKATFFIPSNLAYGPQELPG-IPANSTLIFDVELISVK 2L2S Chain:A ((7-117)) -----------------------------------------------------------------------------------------------------------------------------------VVTTESGLKYEDLTEGSGAEARAGQTVSVHYTGWLTDGQKFDSSKDRNDPFAFVLGGGMVIKGWDEGVQGMKVGGVRRLTIPPQLGYGARGAGGVIPPNATLVFEVELLDV-

General information: TITO was launched using: RESULT: Template: 2L2S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 485 -8871 -18.29 -82.13 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -18.29 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_2L2S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L2S-query.scw PDB file : Tito_Scwrl_2L2S.pdb: