Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYQYHCACCDKV--VASTDKSCPYCGSHHIRSPYGLWMFCVMACLAVVVVFKVVHLYIQNHQDTPVQSTLLDVLNEGDKSTKQ 2N9P Chain:A ((11-41)) --GRYFCHCCSVEIVPRLPDYICPRCESGFIEE---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2N9P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 99 -13878 -140.18 -478.53 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -140.18 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_2N9P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N9P-query.scw PDB file : Tito_Scwrl_2N9P.pdb: