Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSFFPSPRYFFISVVIWLALNMVLWYTGGDHWGQYLGFPQGYADAELPIGVSRFWSPAFLWFYLWFLVSTALFAGFWKIISNNPWQRWSIWGAAFILFNIWFAVQVNVAINAWYVPFWDLIQKMLSSG-G-GD-------------ISTLYSETLVFLYIAMVYVTLGVITAFFT----SHYVFRWRTAMNEYYTEH---WEKLRHIEGASQRVQEDTMRFATIMEDLGVELVKSVITLIAFLPILFHLSKYVLVLPLVGELNHSLVWAAIVWSIFGTVLLMVVGIKLPGLQFNNQKVEAAYRKELVYGEDHAE--RA--QPVTLKELFAN----VRKNYFRLYFHYAYFNMTATWYGQLDILYNLVVLFPSIAAGKLTLGLMQQIMNVFGRVRDSFQYLISSWKTIIELLSIYKRLKAFESILHK 5KO2 Chain:A ((53-394)) -------------------------------------------------------------------------------------------------GTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLT-------------LVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNK--

General information: TITO was launched using: RESULT: Template: 5KO2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 810 -78238 -96.59 -273.56 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -96.59 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5KO2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KO2-query.scw PDB file : Tito_Scwrl_5KO2.pdb: