Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MTKKNNAPELHKYRGLTSTEQMVIHQMLISYVREENCRFNIIMTGTAEPYNLVKLTSINFENEASAIWVHFETITGEQIALPIDFLSRIEFSGQQ--EI--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5IC7 Chain:A ((58-422)) RKLRPETIDIQRTRDIPDTHK----AAISSLAFHP-----------RYPILLSSS--TSSIMYLHKLD----ASAYPTPNPLLTSVHVKRT--DLRRAAFVGPDGGEIIFAGRRRYFHCWNLSSGLVKKVSKIQGHQKEQRTMERFRVSPCGRYMALVASDKKGGGMLNIINVGTMQWIAQARIDGRHGVADFAWWSDGNGLTIAGRDGQVTEWSMITRRTVGIWRDEGTVMALGGRNGPAELGGDRWVAIGSNSGILNVYDRNDLIEIRIKKYPTPTRVFEQLTTSISVVAFSPDGQLLAFGSQHKKDALRLVHLPSCTVYRNWPTEQTPLGRVTAIAFSSKSDVLAVGNDVGRVRLWEIRG

General information: TITO was launched using: RESULT: Template: 5IC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 216 -6417 -29.71 -86.72 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -29.71 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_5IC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IC7-query.scw PDB file : Tito_Scwrl_5IC7.pdb: