Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFASILRELNYLRSNKWDSCMVTLIPAFIIFLFSSMFYAGKAEHLPIAIIDQDQSELSRNIGKYLSLNHTVDVKTITT-SSSEAER-LLNETKIWGYIFIPDGAEKRFVKAQDAQISIAFNQSFFSVGNTISSAMLVSTLNALADYAGQNYLANNIPYLDVPTAHVKISTLYNPSMSYEFYLEPFMVPAVLHLLLCCCVAFAIGQEIKRGTLTQWVNRESFIQGLLAKNLLYSFIFCFWTWLWMFWLVEIRGWFVAGSLSFLLAAQFLLYFSYALISSTVILATKNLSKTFGFIAVYGGSSLSFAGVTLPLNNAPIFTKFWSLIIPYTPYAKLQTEQWVIGSPLFISMSPFLILIGYCLIYFFLSCLLLKKLVQGATP 2P0S Chain:A ((8-68)) ------------------------------------------DMKTIAIADRTGE-YEQLFKE----NDEFRFV-HAEKTAEEYRKMGADKSGIDAVLEIRQD-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 177 -6218 -35.13 -117.31 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.41 3D Compatibility (PKB) : -35.13 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.41 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_2P0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P0S-query.scw PDB file : Tito_Scwrl_2P0S.pdb: