Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKYLFSIFCLGANIHCYATDFGATNNFVSPNLQLKQNILPPTPKNIPLPAFGQRIIGWGTGAEGARQRLENIQPADVSMIKKQGTTLEMITAWQDFYEQEQQRNENNPTAKYRARLMKKIADLW 2MMB Chain:A ((1-107)) ------------------GTDFGTTNNFVSPNLQLKQNVLPPTPKNIPLPAFGQRIIGWGTGAEGARQRLENIQPADVSMIKKQGTTLEMITAWQDFYEQEQQRNENNPTAKYRARLMKKIADLW

General information: TITO was launched using: RESULT: Template: 2MMB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 355 -32150 -90.56 -300.47 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -90.56 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_2MMB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MMB-query.scw PDB file : Tito_Scwrl_2MMB.pdb: