Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVIVLAGTIGAGKSSLT----------EMMAEHFDSQAFYESIDD--------NEVLPLFYANPEQYAFLLQIYFLNKR----FASIKQAMKD-DNNVL--DRSIYEDSLLFHLNA-DLGRATETEVRVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI 4Q1E Chain:A ((44-235)) ---ISIEGNIAAGKSTFVNILKQLSEDWEVVPEPVARWSN------LTMEQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESESMNETEWTIYQDWHDWMNNQF---GQSLELDGIIYLQATPETCLHRIYLRGRNEEQ----GIPLEYLEKLHYKHESW-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q1E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 606 10237 16.89 65.62 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 16.89 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4Q1E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q1E-query.scw PDB file : Tito_Scwrl_4Q1E.pdb: