Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINMEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTTFSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTA-VPGSCTITSGIHVDHISNAQINASFPMSEEL-GYKILDWLEKIDFHIK-EPVYYKDGEKPL 4GZV Chain:A ((4-142)) KTKFKAADLKGIWQLCHYVSESPDVPGALKPSNTFKVLSDD-GRIVNFTIIPGADAIITGYGTYKQLTDDSYKESIEKNIHLPMLDN---QDNILEFEIKDNDYLHLKYFIKSDLNGNELNTWYYETWKRVEMPAKFPEDIVR--

General information: TITO was launched using: RESULT: Template: 4GZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 499 8828 17.69 64.91 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 17.69 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.080

(partial model without unconserved sides chains): PDB file : Tito_4GZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GZV-query.scw PDB file : Tito_Scwrl_4GZV.pdb: