Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDH------LYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGI---QCLSFS--FISYIIVLIFLSFIVWMFYLVKK---------- 1D09 Chain:B ((1-153)) --------MTHDNKLQVEAIK-----RGTVIDHIPAQIGFKLLSLFKLTETDQRITIGLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPERIDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN

General information: TITO was launched using: RESULT: Template: 1D09.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 509 -40482 -79.53 -306.68 target 2D structure prediction score : 0.14 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : -79.53 2D Compatibility (Sec. Struct. Predict.) : 0.14 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.048

(partial model without unconserved sides chains): PDB file : Tito_1D09.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D09-query.scw PDB file : Tito_Scwrl_1D09.pdb: