Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEESMRIACYGVRPNEIDFFEKLNI-YHYDLSLYEELLTHDNIVSAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFH--SLEELLAKSDIVSIHVPHIPGENDQMINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR 3WWY Chain:A ((25-354)) ---QKTKIIFFDIKDYDKEFFKKYGADYNFEMTFLKVRLTEETANLTKGYDVVCGFANDNINKETIDIMAENGIKLLAMRCAGFNNVSLKDVNE-RFKVVRVPAYSPHAIAEYTVGLILAVNRKINKAYVRTREGNFSINGLM-GIDLYEKTAGIIGTGKIGQILIKILRGFDMKVIAYDLFPNQKVADELGFEYVSLDELYANSDIISLNCP-LTKDTKYMINRRSMLKMKDGVILVNTGRGMLIDSADLVEALKDKKIGAVALDVYEEEENYFFED-KSTQVIEDDILGRLLSFY-NVLITSHQAYFTKEAVGAITVTTLNNIKDFVEGRPLVNEV---------

General information: TITO was launched using: RESULT: Template: 3WWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1681 -13556 -8.06 -41.45 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -8.06 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_3WWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WWY-query.scw PDB file : Tito_Scwrl_3WWY.pdb: