Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSGVATLKKYFDGSVVPENP-----RITNAQKSIRT----NDIENVGKTARHHTMFEMLGNFSIGDYFKNEAIHWAWEFLTDPKWMAFDPEKLYVTVY--------PKDTEAKRIWHEEVGLPLDHII--DVEDNFWDIG-AGPCGPDTEIFYDR---GEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTFEPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVT-----------DISYKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHG-KKLGIEQAFLYRLVPVVGEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGKDIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSALLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQVADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIKNHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKIWEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIGIFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQLQQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQWKQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMAQAGGKNPAGIPDALKAAKEWVAQQ 4XEO Chain:A ((25-408)) ---LTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHN-LDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLT--QEFGIPIERLYVTYFGGDEAAGLEADLECKQIW-QNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN---------VLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGT-----GARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILHRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4XEO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1916 49031 25.59 140.89 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 25.59 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_4XEO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XEO-query.scw PDB file : Tito_Scwrl_4XEO.pdb: