Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNMYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKNVIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS----PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHVESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGNIRVKHFVSDSNDSAKDQALKFLEA---------GDKMKKWIMRHHHQLLITSGMI-----------ELLALYQQQKFPG-----LGVLKKWSLMHHL---ELISHLLDHPKDW--------LKYYSKVQELYEVIQ------- 1FUI Chain:A ((1-355)) MKKISLP-----KIGIRPVIDG-RRMGVRESLEEQTMNMAKATAALLTEKLRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPTRPKAIWGFNGTERP----GAVYLAAALAAHSQKGIPAFSIY-GHDVQDADDTSIPADVEEKLLRFARAGLAVASMKGKSYLSLGGVSMGIAGSIV-DHNFFESWLGMKVQAVDMTELRRRIDQ-KIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQGNSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVVATENDSLNGVAMLMGHQLTGT

General information: TITO was launched using: RESULT: Template: 1FUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1326 -25267 -19.05 -82.03 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -19.05 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_1FUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUI-query.scw PDB file : Tito_Scwrl_1FUI.pdb: