Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDNKHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEEFSQEEHLVFICGHYEGYDER-IRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVLGNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQRRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK 4YQ9 Chain:A ((9-251)) MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAA-GEGAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVLG--------SFADGLLDCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQAE----------

General information: TITO was launched using: RESULT: Template: 4YQ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1000 -94032 -94.03 -401.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -94.03 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_4YQ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YQ9-query.scw PDB file : Tito_Scwrl_4YQ9.pdb: