Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRES--------SFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLYW--------------VVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK 5I6Z Chain:A ((165-306)) -----------------------------------------------------------------------------------------------------------------------------QIHRSKGLQDLGGISWQLALCIMLIFTVIYFS--IWKGVKTSGKVVWVTATFPYIAL---SVLLVRGATLPGAWRGVLFYLK-PNWQ-KLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVI---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5I6Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 101 -10262 -101.60 -85.52 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -101.60 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : -0.118

(partial model without unconserved sides chains): PDB file : Tito_5I6Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I6Z-query.scw PDB file : Tito_Scwrl_5I6Z.pdb: