Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQ--------PKQYEYSRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHA-VFSLFQAGDHILLGDDVYGGTFRLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLAHQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQIGF--YQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPGLPDHELHGVAKQQMSGFSGMISFTLKNE-ESAIPFVESLQLFTLAESLGGVESLVEIPSVMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN 5M3Z Chain:A ((11-395)) ----TQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARFALEESGYIYTRLGNPTTDALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYGHTHAFLSHSMPKFGINVSFVDAAKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDNTFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGK-QEFIDQARFVGLKDITGGCMSPFNAWLTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLSSHPQYELGQRQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEERLKAGITDGLIRLSVGLEDPEDIINDLEHAIRK---

General information: TITO was launched using: RESULT: Template: 5M3Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2176 9308 4.28 24.95 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 4.28 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_5M3Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M3Z-query.scw PDB file : Tito_Scwrl_5M3Z.pdb: