Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQIIDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHGRVQALAEAARENGLEF 2ZJQ Chain:L ((26-111)) ---------------------------RLRLSVYRSSKHIYAQIIDDSRGQTLAAASSAALK--SGNKTDTAAAVGKALAAAAAEKGIKQVVFDRGSYKYHGRVKALADAAREGG---

General information: TITO was launched using: RESULT: Template: 2ZJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 409 -25555 -62.48 -297.15 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain L : 0.82 3D Compatibility (PKB) : -62.48 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_2ZJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZJQ-query.scw PDB file : Tito_Scwrl_2ZJQ.pdb: