Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVSAFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTNVLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKF-----A-TAVNIASAPTGMVIPPST-AFIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVIIIGG-ILTGIFTA-----VE-ASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQSVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGV-T-DNKFIILLLVNLVLLLVGMFMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGGVKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS 5ULD Chain:C ((38-440)) -----------IAVLWLTEALHVTVTAILVPVMAVFFGI---FE----TQAALNNFANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSMWI-SNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGTGPNAIAAAEVG-LSFTDWMKFGLPTAMMMLPMAIAILYFLLKPTLNGMFELD--RAPVNWDKGKVVTLGIFGLTVFLWIFSSPINAALGGFKSFDTLVALGAILMLSF--ARVVHWKEIQKTAD------WGILLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFTSNVATTTLLIPVFATVAEAFGMSPVLLSVLIAVAASCAFMLPVATPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIA--------------

General information: TITO was launched using: RESULT: Template: 5ULD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2059 -298420 -144.93 -771.11 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -144.93 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_5ULD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ULD-query.scw PDB file : Tito_Scwrl_5ULD.pdb: