Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHIELKNKIVFVTGVAGFIGSNLTKRLFADIEGVKVVGIDNMNDYYDVRLKEARLEELFKFENFVFVKGNLADKAVITAIFEEHHPNIVVNLGAQAGVRYSIINPDAYIEGNMIGFYNILEACRHYPVEHLVYASSS-SVYGSNKKIPYSTDDKVDNPVSLYAATKKSNELMTHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYFG-------FTNKLLKGETIQIFNYGNCKRDFTYIDDIVEGVIRIMQGAPEKQTGEDGLPVPPYAVYNIGNHNPENLLDFVQILLEELIRAGVLPEDYDFEAHKELVPMQPGDVPVTYADTEALERDYGFKPATPLRTGLRKFAEWYKEFYMSETV 4ZRN Chain:A ((6-309)) ----------ILVTGGAGFIGSHVVDKLIEN--GYGVIVVDN--------LSSGKVENLNR--NALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVREPARDAKTNIIGSLVLLEKSIKYGVKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDP----YGEAGVVAIFTERMLRGEEVHIFGDGEYVRDYVYVDDVVRANLLAM----EKGDNE---------VFNIGTGRGTTVNQLFKLLKE--------ITGYDKEPVYK--PPRKGDVRKSILDYTKAKEKLGWEPKVSLEEGLKLTVEYFRK-------

General information: TITO was launched using: RESULT: Template: 4ZRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1713 -48501 -28.31 -163.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -28.31 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_4ZRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRN-query.scw PDB file : Tito_Scwrl_4ZRN.pdb: