Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYKGLKIAVAGTGYVGLSIATLLAQSHT---VTAVDIIPEKVELINNKKSPIQDDYI-EMYLAKKELDLMATVDGVSAYKDADFVVIAAPTNYDS----KKNFFDTSAVEAVIK-LVMEVNPEAIMVIKSTIPVGYTASIR-------EKTGSKNIIFSPEFLRESKALYDNLYPSRIIVGTDMEDERLVEAAQTFAKLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQQIINGVCRDPRIGTHYNNPSFGYGGYCLPKDTKQL--LANYEDVPE--NLIEAIVESNRTRKNFIADRVLELAGAYEANSHWDASKEKKVVIGVYRLTMKSNSDNFRQSSIQGVMKRVKAKGTTVVIYEPSLKDGEIF--FGSKVVNDLDQFKAMCDSIIANRYDTCLDDIKEKVYTRDIFQRD 5VR8 Chain:A ((7-406)) -------KICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGD-ETPEGQRAVQALCAVYEHWVPREKI--LTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIID--------SLFNTVTDKK--IAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSH-----DDQVSRLV-TISKDPYEAC-----------------

General information: TITO was launched using: RESULT: Template: 5VR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1889 15899 8.42 42.62 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 8.42 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_5VR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VR8-query.scw PDB file : Tito_Scwrl_5VR8.pdb: