Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGV--AVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILE---ERKARDINDYSYEEVERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIG---G-QASTKEMMEAIIARL 3U1H Chain:B ((2-355)) -KKKIAVLPGDGIGPEVMEAAIEVLKAVAERFGHEFEFEYGLIGGAAIDEAGTPLPEETLDVCRGSDAILLGAVGGPKWDQNPSELRPEKGLLGIRKGLDLFANLRPVKVYDSLADASPLKKEVIEGVDLVIVRELTGGLY-------EEGEEAAVDTLLYTREEIERIIRKAFELALTRKKKVTSVDKANVLESSRLWREVAEEVAKEYPDVELEHMLVDNAAMQLIRNPRQFDVIVTENMFGDILSDEASMITGSLGMLPSASLSTDGLGLYEPVHGSAPDIAGKGIANPLATILSAAMMLRYSFGLEEEAKAIEKAVEKVLAEGYRTADIAKPGGKYVSTTEMTDEVKAAV

General information: TITO was launched using: RESULT: Template: 3U1H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1880 -1423 -0.76 -4.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -0.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_3U1H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U1H-query.scw PDB file : Tito_Scwrl_3U1H.pdb: