Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------MLFRAMANKKLLICAAIAAGLLLTACVKKETPKEEEQDKVETAVSEPQPQKPAKFESLESVDTQETQVQEQPQVEVHREETANTTTEIRRETRPARSDESSQTQVAEQPKSETP----------KVEPKPEKKPEPKAEPKPEKAQ-SKPAAKATEPANTEDDAVAAAIAAATPALKN----------------------------------------------------------------------------------- 5X5Y Chain:F ((1-362)) MIVFRYLSREVLVTMSAVSAVLLVIIMSGRFIKYLAQAAQGLLDPGSLFLIMAFRIPGFLQLILPLGLFLGILLAYGRLYLESEMTVLSATGMSQKRLLGYTMAPALLVAILVAWLSLFLAPQGINQFALLLNKQDT-LTEFDTLVPGRFQAMRD-GTRVTYTEELSK---DRGELAGIFISQKDLSNQERGISILVAEKGTQNIQADGSRYLILHNGYRYDGNPGQANYRAIQYDTYGVMLPKPDAVPTADLFGSDNPRYQAELQWRLSTPLLVFVVTLLAVPLSRVNPRQGRFLKLLPAILLYMGYLALLIAVRGQLDKGKIPMAIGLWWVHGLFLAIGLLLFYWEPLRLKLAS

General information: TITO was launched using: RESULT: Template: 5X5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 440 46543 105.78 287.30 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain F : 0.46 3D Compatibility (PKB) : 105.78 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_5X5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X5Y-query.scw PDB file : Tito_Scwrl_5X5Y.pdb: