Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLAVFFNKRVYNIPVRFIEATKQYALGRKKNGEEYFGSVAEIIRNH 2CRH Chain:A ((28-102)) WYAGPMERAGAESILA-NRSDGTFLVRQRVKD--AAEFAISIKYNVEVKHIKIMTA--EGLYRITE----KKAFRGLTELVEFY

General information: TITO was launched using: RESULT: Template: 2CRH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 282 -43929 -155.77 -585.71 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -155.77 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_2CRH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CRH-query.scw PDB file : Tito_Scwrl_2CRH.pdb: