Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRRKSVLYFYDENIGNFH--YGPSHPMKPHRVRMTHDLIQAYDLLQSMDIVKPLKPAVEDLTRFHANDYVDFLRASAQSRLEASLSPRPFLFFSPSPAAARSASRPSPPPQAEGAAAVSAGASGVSVVQSGSGSRVRSTLHPLRSESRSVSPATSTFDSRVPPEAVGADALSSTDARGRTPPKSLGVDLGPGGWSRAATQKPPFLQASSGDGAHGPLGLQCPSAAEETGTGGQAPAWAPPGVSPSLRRGEDANGLLSSAAFDEEVAAPPVPLSSSLADQMARFNVGEDCPVFDGLWEYCQTYSGGSIEGARRVVRGEYDWAIN-WAGGLHHGKKHEASGFCYINDCVLAALEFLRF---KHRVLYVDVDIHHGDGVEEAFYTSPRVLCCSFHKYG--DYFPG--TGALDDVGIDEGLGYSVNVPLS-EGVDDAMYTRLFRQVMDMIMESYRPEAVVLQCGADSLSGDRLGCFNLSIDGHSEAVRYFCSK-DVPALFLGGGGYTLRNVPRCWATETGYVLGLSLNPNIPEESEYVGYYGPDSTLHVRTSNMENRNEDSYVQHVIERISQTLKDHVRPVGAQISANYFLDDSVMDKSDQIRALLADEEEEKAMDSSGDAEDADGAGRFEEISNRNRG 5EDU Chain:B ((388-745)) ---ASRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRE-L-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HRESSN---FDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPL-LT--------------------RPPLSGALASITETIQVHRRYWRSLR-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EDU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2202 -40317 -18.31 -117.20 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -18.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_5EDU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EDU-query.scw PDB file : Tito_Scwrl_5EDU.pdb: