Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFIKVAAAVIKKDDLYLCARRKENKYKYLSKKFEFPGGKVESGETLQEALVREIYEELGVKVCINNELKKVQHEYPDFKVEITFFSCNFVGNYQYVNFDHEEIIWLPAAELALLDWAAADLPIVDLLQQI 3FFU Chain:A ((26-144)) -------AGFLRKDGKILVGQRPENN--SLAGQWEFPGGKIENGETPEEALARELNEELGIEAEVGELKLACTHSYGDVGILILFYEILYWKG-EPRAKHHMMLEWIHPEELKHRNIPEANRKILHKIY--

General information: TITO was launched using: RESULT: Template: 3FFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 503 -54011 -107.38 -453.87 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -107.38 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_3FFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FFU-query.scw PDB file : Tito_Scwrl_3FFU.pdb: