Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLAVFFNKRVYNIPVRFIEATKQYALGRKKNGEEYFGSVAEIIRNH 2IUG Chain:A ((12-87)) WYWGDISREEVNEKLR-DTADGTFLVRDASTKM-HGDYTLTLRKGGNNKLIKIFHR--DGKYGFSD----PLTFSSVVELINHY

General information: TITO was launched using: RESULT: Template: 2IUG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 275 -36065 -131.15 -474.54 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -131.15 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_2IUG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IUG-query.scw PDB file : Tito_Scwrl_2IUG.pdb: